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【Objective】 To explore the intention of voluntary blood donation in students from a middle vocational school in Jinhua and its influencing factors. 【Methods】 A total of 400 students were enrolled from a middle vocational school in Jinhua by convenience sampling, and a self-designed questionnaire was issued to each student to investigate the cognition, intention and attitude of voluntary blood donation. The influencing factors of voluntary blood donation intention were analyzed by Logistic regression. 【Results】 A total of 394 valid questionnaires(98.50%) were collected. For 394 surveyed students, the average score of cognition of voluntary blood donation was (7.27±2.69) points, and the overall rate of intention to voluntary blood donation was 21.32%. Logistic regression analysis equation consisted of family support, better cognition, feeling happy about blood donation and worrying about blood donation, with risk ratios(OR) as 31.78, 188.69, 26.27 and 0.01, respectively(all P<0.05). 【Conclusion】 Students from a middle vocational school showed poor congnition and intention of voluntary blood donation.Family support for voluntary blood donation, better cognition of voluntary blood donation and happiness for blood donation were positive factors, and the fear of blood donation was a negative factor.It is necessary to strengthen the publicity of blood donation among students in middle vocational school to improve their congnition and intention to voluntary blood donation.
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【Objective】 To explore how the awareness and prevention status of COVID-19 epidemic, as well as anxiety affect blood donation behaviors of college students. 【Methods】 From June 7 to 13, 2020, students from two colleges in Jinhua were given self-made questionnaires through Wenjuanxing (a platform providing functions equivalent to Amazon Mechanical Turk), general demographic information, voluntary blood donation, core knowledge and prevention measures of COVID-19, as well as general anxiety were investigated. 466 valid questionnaires were collected, and the influencing factors of voluntary blood donation were analyzed by Logistic regression. 【Results】 College students donated(88.1±19.8)% were more likely to conduct qualified protections against COVID-19 than non-donation students(83.3±19.0)%(P<0.05), and be less anxious (9.68±4.80 vs 10.86±4.74, P<0.05). During COVID-19 outbreak, male donors was 3.732 times females(P<0.05). The donation rate of students with good awareness of COVID-19 protection, getting 60.0% correct or more, was 4.663 times students getting 60.0% below correct (P<0.05), and students with anxiety total score below 10 points was 2.227 times those above 10 points(P<0.05). 【Conclusion】 Gender, awareness of protections against COVID-19, and anxiety level are important factors affecting the blood donation behaviors of college students during COVID-19 epidemic, therefore strengthening psychological counseling and improving protection measures against COVID-19 are conductive to promote blood donation of college students during COVID-19 epidemic.
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Liver nonparenchymal cells play an important role in hepatitis B virus (HBV)-related liver diseases, and the activation of hepatic stellate cells (HSCs) and their interactions with intrahepatic cells are the main cause of liver fibrosis. This article mainly introduces HBV-induced liver inflammation and the interaction between innate immune cells and HSCs and briefly describes the role of monocytes/macrophages and natural killer cells in the activation and killing of HSCs and the immunomodulatory effect of HSCs in the presence of HBV.
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Objective:To explore the effect of oral motor intervention on oral feeding ability and growth of neonates with intestinal atresia.Methods:A total of 80 intestinal atresia neonates in the Hunan Children`s Hospital from January 2017 to January 2019 were admitted to the present study. Neonates were randomly assigned to oral motor group and control group according to the random number table. The control group received routine nursing, while the oral motor group carried out oral motor intervention for 14 consecutive days. Both groups were followed up for six months. The oral feeding ability and growth index such as body weight, body length and head circumference were compared between two groups.Results:The complete oral feeding rate transfer, complete oral feeding rate transfer, time to complete oral feeding were (10.48±2.96) ml/min, (90.02±8.36) %, (15.06±2.99) days in the oral motor group, those index were (8.18±2.44) ml/min, (72.58±9.46) %, (18.08±4.98) days in the control group, the differences were statistically significant ( t values were 3.633, 8.316, 3.106, P<0.01). After 7, 14 days of intervention, the non-nutritive sucking scores were (52.24±8.89) points, (69.81±12.94) points in the oral motor group, significantly higher than (48.08±6.72) points, (63.09±11.73) points in the control group, the differences were statistically significant ( t values were 2.265, 2.327, P<0.05). After 3, 6 months of intervention, the levels of body weight were (6 234.21±560.25) g, (7 630.19±782.25) g, significantly higher than (5 916.89±462.40) g, (7 293.65±979.98) g in the control group, the differences were statistically significant ( t values were 2.648, 2.148, P<0.05). After 6 months of intervention, the levels of head circumference were (43.81±5.59) cm in the oral motor group, significantly higher than (40.85±3.73) cm in the control group, the difference was statistically significant ( t value was 2.635, P<0.05). Conclusion:Oral motor intervention can promote oral feeding ability and improve growth performance of neonates with intestinal atresia.
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Hepatitis B virus (HBV) is a member of hepadnavirus, and HBV infection may not cause direct hepatocyte lesions. Host immune response determines whether the virus can be eliminated and is a major cause of HBV-related liver diseases. Myeloid-derived suppressor cells (MDSCs) are a group of non-lymphoid immune suppressor cells originating from bone marrow and can regulate innate and adaptive immune responses under different pathological conditions. Studies have shown that chronic hepatitis B patients have a significantly higher number of MDSCs in peripheral blood than the healthy population. This article introduces the role of HBV-induced MDSC subsets in persistent HBV infection and liver pathological mechanism.
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Objective To discuss the effect ofrivaroxaban in preventing deep venous thrombosis after large orthopedic operation of lower extremity.Methods Totally 80 patients after lower extremity of large orthopedic operation were selected,they were divided into two groups randomly.The patients in control group (39 cases) were sc injected with enoxaparin through.The patients in observation group (41 cases) were given rivaroxaban orally.The effect of rivaroxaban in preventing deep venous thrombosis after large orthopedic operation of lower extremity were evaluated by hemodynamic indexes,diagnosis index,and complications during 1 months follow-up.Results Before operation,there was no statistical significance on platelet,hemoglobin,and PT values between two groups.Compared with the value before operation,the value ofplatelet and hemoglobin had no significantly change after 3 d of operation.But the PT was increased (P ＜ 0.05).After 3 d of operation,there was no statistical significance on platelet and hemoglobin between two groups.But the PT value of observation was higher than that of control group (P ＜ 0.05).After 1 h operation,there was no statistical significance on D-dimer,Hcy,and hs-CRP between two groups.Compared with the value after 1 h of operation,the values of D-dimer,Hcy,and hs-CRP were decreased significantly (P ＜ 0.05).And the indexes of observation group were lower than those of control group (P ＜ 0.05).After 1 months of operation,although there was no statistical significance on complications between two groups,the incidence rate of secondary bleeding and DVT of control group was higher than that of the observation group.Conclusion The rivaroxaban could prevent DVT well,which is suitable for the anticoagulation therapy after lower extremity of large orthopedic operation with little influence on platelet and hemoglobin.It is worthy of clinical application.
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Objective To investigate the influence of glycyrrhizic acid (GA) on oxidative stress injury in glomerular mesangial cells (HBZY-1) induced high glucose.Methods HBZY-1 was cultured and divided into the normal control group,high glucose group and high glucose +-GA group.The cell proliferation activity was measured by MTT assay.UV spectrophotometry was used to detect the SOD and MDA levels.And the ROS changes were detected by laser confocal microscopy.Immunohistochemistry and Western blot were used to detect the expression of Mn-SOD protein in various groups.Mn-SOD mRNA was detected by Q-PCR.Results (1) The morphological changes in each group:HBZY-1 cells were diamond-shaped,cells were normal and the structure was clear in the NG group.In the HG+-GA group,the number of cells was increased slightly,individual cell body was slightly hypertrophy.The cell structure in the HG group was not clear,the cells appeared flat,the number was increased significantly,the cell body was slightly hypertrophy.(2)The proliferative effect of HBZY-1 in each group:compared with the NG group,the OD value in the HG group was significantly increased,and the OD value in the HG+GA group was decreased compared with the HG group(P＜0.05).(3)The relative content of ROS in the HG group was higher than that in the NG group,and which in the HG+GA group was decreased (P＜0.05).(4)The expression of Mn-SOD in each group:the relative expression of Mn-SOD in the HG group was significantly lower than that in the NG group (P＜0.05),and the expression of Mn-SOD in the HG+-GA group was increased compared with the HG group(P＜0.05).Conclusion Glycyrrhizic acid has a certain inhibiting effect on the abnormal proliferation of HBZY-1 induced by high glucose.Glycyrrhizic acid can protect the cell hypertrophy and cell damage caused by glomerular mesangial cells via oxidative stress,and glycyrrhizic acid can regulate the Mn-SOD expression induced by high glucose.
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Objective To investigate the influence of glycyrrhizic acid (GA) on oxidative stress injury in glomerular mesangial cells (HBZY-1) induced high glucose.Methods HBZY-1 was cultured and divided into the normal control group,high glucose group and high glucose +-GA group.The cell proliferation activity was measured by MTT assay.UV spectrophotometry was used to detect the SOD and MDA levels.And the ROS changes were detected by laser confocal microscopy.Immunohistochemistry and Western blot were used to detect the expression of Mn-SOD protein in various groups.Mn-SOD mRNA was detected by Q-PCR.Results (1) The morphological changes in each group:HBZY-1 cells were diamond-shaped,cells were normal and the structure was clear in the NG group.In the HG+-GA group,the number of cells was increased slightly,individual cell body was slightly hypertrophy.The cell structure in the HG group was not clear,the cells appeared flat,the number was increased significantly,the cell body was slightly hypertrophy.(2)The proliferative effect of HBZY-1 in each group:compared with the NG group,the OD value in the HG group was significantly increased,and the OD value in the HG+GA group was decreased compared with the HG group(P＜0.05).(3)The relative content of ROS in the HG group was higher than that in the NG group,and which in the HG+GA group was decreased (P＜0.05).(4)The expression of Mn-SOD in each group:the relative expression of Mn-SOD in the HG group was significantly lower than that in the NG group (P＜0.05),and the expression of Mn-SOD in the HG+-GA group was increased compared with the HG group(P＜0.05).Conclusion Glycyrrhizic acid has a certain inhibiting effect on the abnormal proliferation of HBZY-1 induced by high glucose.Glycyrrhizic acid can protect the cell hypertrophy and cell damage caused by glomerular mesangial cells via oxidative stress,and glycyrrhizic acid can regulate the Mn-SOD expression induced by high glucose.
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Molecular imprinting technique (MIT) involves the synthesis of polymer in the presence of a template to produce complementary binding sites in terms of its size, shape and functional group orientation. Such kind of polymer possesses specific recognition ability towards its template molecule. Despite the rapid development of MIT over the years, the majority of the template molecules that have been studied are small molecules, while molecular imprinting of proteins remains a significant yet challenging task due to their large size, structural flexibility and complex conformation. This review, we summarized the research findings over the past years, and discussed the nano-reinforcing materials used to prepare molecular imprinting of proteins and the perspective of these nano-reinforcing materials.
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BACKGROUND:Studies about low-frequency pulsed electromagnetic fields interfering with bone marrow mesenchymal stem cells proliferation and differentiation are many, but the Raman spectra of single stem cells irradiated in electromagnetic fields analyzed by surface Raman spectroscopy analysis are rarely reported. OBJECTIVE:To compare the difference in Raman spectra of bone marrow mesenchymal stem cells with or with no irradiation of 3 000 Hz pulsed electromagnetic fields. METHODS:Bone marrow mesenchymal stem cells isolated from Sprague-Dawley rats were cultured and identified. Passage 3 cells were inoculated into 6-wel plates and divided into two groups:pulsed electromagnetic field irradiation group and blank control group. After cultured for 7 days, cells in the two groups were transferred to physiological saline, and 30 cells were randomly col ected from each group. Four Raman spectra were harvested from each celland the average relative intensity of Raman spectra was calculated and compared between two groups. RESULTS AND CONCLUSION:There were the same Raman peaks in the two groups, and the waveforms were basical y same in the two group based on the curve mapping by origin 7.0 software. The peak value in the irradiation group was decreased compared with the blank control group. Laser optical tweezers Raman spectroscopy can be applied to study the biochemical changes of a single stem cellat the molecular level. The Raman spectra of bone marrow mesenchymal stem cells irradiated by 3 000 Hz pulsed electromagnetic fields differ from those without irradiation, and the peak also lowered after irradiation.
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To investigate the effect of low power helium neon laser (He-Ne laser) on the telomere length of human fetal lung diploid fibroblast (2BS) cell, we used the laser (gamma = 632. 8 nm, P = 2 mW) to treat the young 2BS cells. Cell growth and proliferation was observed through MTT method after treating with low power laser. The relative telomere length of 2BS cells was detected by fluorescence real-time quantitative PCR (q-PCR). The results showed that the cells of the treated groups grew better than the untreated groups. The telomere DNA length of the old 2BS cells, treated by low power He-Ne laser when they were young, was longer than that of untreated group. The results of the present study indicated that the low power He-Ne laser might decrease shortening rate of telomere and delay the aging of cells. Therefore, this study provides the experimental basis for us to further investigate the effect of low power laser on cell aging at the gene level.
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Humans , Cell Line , Cellular Senescence , Radiation Effects , Fetus , Fibroblasts , Cell Biology , Lasers , Lung , Cell Biology , Telomere Homeostasis , Radiation EffectsABSTRACT
Objective To summarize the keypoints of clinical diagnosis of chondroblastoma and the experience on the treatment Methods The clinical materials of 16 cases(11 males,aged from 12 to 26 years)from June 1992 to February 2003 of pathologically diagnosed chondroblastoma were analyzed retrospectively. The tumor located at distal femur in 6 cases,great trochanter in 1 case,proximal tibia in 6 cases,proximal humerus in 2 cases, talus in 1 case. The main symptoms were swelling and painful joint. All the 16 cases were treated with curettage and autologous bone graft from iliac crest. Results Twelve cases were followed up for 6 months to 5 years .averaging 3. S years. All the bone graft healed well. One case., recurred 2 years after the initial curettage and was treated with curettage again. No recurrence occurred at the last follow-up 6 months after the second curettage. The function of the involved joints was nearly normal in all cases except for one case with slight limitation of knee flexion. Conclusions Mastering the image and clinical characteristics of chondroblastoma can avoid misdiagnosis and mistreatment Curettage can control the tumor satisfactorily. Postoperative rehabilitation can improve the function of the involved limb.
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Objective: To introduce the Raman spectrum, the optical tweezers physical principle and its working characteristics, unify the detail medical research work, summarize specific application situation of the Raman tweezers technology in the different cells domain. Methods: Use the optical tweezers to fix the living cells, simultaneously carry out Raman spectrometry on the living cell or the cell organ by using the laser Raman technology. By applying this technology, the samples will be captured in the suspending liquid. In an approximate physiological state, the single living specimens, such as the cells, the cell organs or the biological macro-molecules, will be studied and the real-time track to the research object physiological biochemistry process will be carried on, then the Raman spectrometry will be implemented to the living cells. Results: From the single cell level, Raman tweezers technology analyzes the oxygen ability and the deformability of red blood cells of normal persons and the Mediterranean Sea anemia patients, and implements the appraisal of blood red cell and the blood platelet of different species. The Raman tweezers technology reveals the differences between the organizational structure of the cancer cells and that of normal cells in the molecular level, providing important information and data for the cancer diagnosis and the mechanism analysis. The Raman tweezers technology has implemented the torsion and knotting of DNA molecules, and realizes the control and differentiation of human being's chromosome. Conclusion: The Raman tweezers technology is the prompt and effective tool for the real-time research of cell physiology and biochemistry changes, hopefully in the molecular level. It will become one of the most advanced tools to carry out examination and diagnosis of different kind of living cell. Surely it has a very bright prospect.
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Traditional teaching mode of biochemistry experiment couldn’t satisfy the demand of cultivating creative medical talents in new era. Offering designing experiment is the developing direction of experiment teaching reforms. But in the practice,there are various of obstacles which prevent the reforming process.
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Objective To improve the diagnosis of CT for osseous abnomalities of lumbar posterior margin concurrent herniated disk.Methods X-ray and CT scan materials of 56 cases with herniated intervertebral disks were retrospectively studied.Results The morbidity of osseous abnomalities of lumbar posterior margin included:lumbar posterior marginal intraosseons node、lumbar posterior marginal osteophytic formation,herniated disk calcification,post longitudinal ligmentum calcification,lumbar positerior marginal fracture.Conclusion This kind of osseous abaomality is a bony fragment that is seen to protrud into the spinal canal,compression of the dural sac and nerve root,concurrent displacement of intervertebral disk.Analysis in combination with X-ray and CT images of coronal or sagittal reconstruction is helpful increasing the accuracy rate of diagnosis.
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AIM: To estabolish the method for quality control of Anxinkang Dripping Pills(Radix Sophorae Flavescentis,Radix Astragali,etc.) METHODS: Using TLC to identify the dripping pills,matrine and oxymatrine in dripping pills were determined by RP-HPLC.Using C_(18) column,the mobile phase was made from the acetone methanol-H_3PO_4(3∶7∶90),the detection wavelength was at 210 nm. RESULTS: The herbs could be(identified) by TLC.For matrine,the linear range was from 0.05-0.50 ?g,and the average recovery was 97.3%,and for oxymatrine,the linear range was from 0.1-1.0 ?g,and the average recovery was 97.6%. CONCLUSION: The method is simple,reliable,accurate and can be applied to the quality control of the Anxinkang Dri-(pping) Pills.
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AIM: To design,prepare and screen out functional small interfering RNA(siRNA) for specifically silencing proliferating cell nuclear antigen(PCNA) gene expression and effectively inhibiting cell proliferation on human hepatoma cell line SMMC-7721,human gastric carcinoma cell line SGC-7901 and human colorectal carcinoma cell line Caco2.METHODS: PCNA siRNA was designed based on previous studies about design guidelines and synthesized in vitro by T7 Mega short script reaction kit according to the manufacturer's instructions.After purification,the integrities of siRNA were identified through 19% denaturing polyacrylamide gel electrophoresis,and the concentrations of the generated siRNA were measured by testing the absorbance at 260 nm using a spectrophotometer.Four synthesized double-strand siRNA were transfected into three types of carcinoma cell lines by LipofectamineTM2000 reagent,respectively.WST-8 assay was employed to examine the proliferative inhibitions of these three cell lines.The subsequent alterations on PCNA mRNA and protein levels were determined by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) and immunocytochemistry,respectively.RESULTS: 3 sequences,No.2,No.3 and No.4 PCNA siRNA showed effective inhibitions on tumor cells among the 4 candidate siRNA,and a single dose of 50 nmol/L of No.2,No.4 PCNA siRNA showed the most effective inhibitory rates as more than 62% at 48 h after transfection.50 nmol/L of No.2 and No.4 PCNA siRNA transfection caused 72% decrease of PCNA mRNA level and almost completely loss of the protein in human Caco2 cells.CONCLUSION: No.2 and No.4 PCNA siRNA have been screened out in this study,which show the capability to effectively down-regulated PCNA expression and significantly inhibit the proliferation of carcinoma cells.The optimal concentration is 50 nmol/L and satisfactory effects are achieved 48 h after transfection in vitro.